Phosphomannose Isomerase from Escherichia coli, recombinant, expressed in E. coli, ammonium sulfate

Stock Code: 3581021
Manufacturer Part No: P2621-10MG
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Application


PMI is used to study cell wall synthesis and energy production. PMI has been used to study how EDTA and metal ions, such as Zn++, Co++, Fe++, Mn++ and Cu++., can affect recovery and thermal stability. It may be used to study PMI?s effect on various alginate biosynthetic enzymes such as phosphomannomutase (PMM), GDP-mannose pyrophosphorylase (GMP), and GDP-mannose dehydrogenase (GMD).


Biochem/physiol Actions


Phosphomannose Isomerase (PMI) catalyses the interconversion of mannose 6-phosphate (Man-6-P) and fructose 6-phosphate (Fru-6-P), which provides a link between glucose metabolism and mannosylation.


Packaging


Bottomless glass bottle. Contents are inside inserted fused cone.


Physical form


Supplied as a suspension in 3.2 M ammonium sulfate


Unit Definition


One unit will convert 1.0 µmole of D-mannose 6-phosphate to D-fructose 6-phosphate per min at pH 7.6 at 25 °C, using a coupled enzyme system with phosphoglucose isomerase and glucose-6-phosphate dehydrogenase.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formammonium sulfate suspension

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