Phospholipase D from Streptomyces sp., Type VII, lyophilized powder, >=150 units/mg solid

Stock Code: 3581100
Manufacturer Part No: P4912-250UN
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Analysis Note


Protein by biuret


Application


Phospholipase D (PLD) has been used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to yield the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD has been used in purification and kinetic studies.


The enzyme from Sigma has been used in the translocation of sphingosine kinase 1 (SK1) to membrane fractions under in vitro conditions. It has also been used to produce phosphatidic acid (PA) from phosphatidylcholine (PC) in HL60 permeabilized cells.


Biochem/physiol Actions


Phospholipase D is glycerophospholipid-specific. It is markedly less active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.


Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.


Packaging


Bottomless glass bottle. Contents are inside inserted fused cone.


Unit Definition


One unit will liberate 1.0 µmole of choline from 1,2-Dioleoyl-sn-glycero-3-phosphocholine per hour at pH 8.0 at 37 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formlyophilized powder

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