Phosphatase, Alkaline shrimp, >=1,000 DEA units/mL, buffered aqueous glycerol solution, recombinant,

Phosphatase, Alkaline shrimp, >=1,000 DEA units/mL, buffered aqueous glycerol solution, recombinant,

Stock Code: 3578925
Manufacturer Part No: A2237-1KU
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Analysis Note


Protein determined by biuret


Application


Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.


Biochem/physiol Actions


Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.


General description


Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.


Physical form


solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2


Unit Definition


One unit will hydrolyze 1 µmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formbuffered aqueous glycerol solution

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