Phosphatase, Acid from potato, lyophilized powder, 3.0-10.0 units/mg solid

Stock Code: 3579972
Manufacturer Part No: P1146-200UN
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Application


The activity of potato acid phosphatase in various surfactant medias was examined. Bis(2- ethylhexyl)sodium sulfosuccinate, Brij 35, and SDS at 3mM inhibited phosphatase activity while the surfactant cetyltrimethylammonium bromide enhanced activity.


Acid phosphatase from potato has been used in a study to assess the potential allergenicity of novel gene products. It has also been used in a study to remove eight phosphate groups from casein at a pH of 7.0.


Biochem/physiol Actions


Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.


General description


Acid phosphatase from potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points.


Packaging


50, 200, 1000 units in poly bottle


Unit Definition


One unit will hydrolyze 1.0 µmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Quality Level300
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formlyophilized powder

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