Peroxidase from horseradish, lyophilized, powder, beige, approx. 150 U/mg

Stock Code: 3578883
Manufacturer Part No: 77332-100MG
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£122.85 (exc VAT) per 100MG
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Application


Peroxidase from horseradish has been used:for isolation of N-Linked glycansas a constituent of D-amino acid oxidase (DAAO) bufferto decompose hydrogen peroxide (H2O2) into water to demonstrate the performance of the portable oxidative stress sensor (POSS)


Horseradish peroxidase is used widely within biochemistry as a detection reagent in a variety of applications including ELISA, immunoblotting, and immunohistochemistry.


General description


Horseradish peroxidase (HRP) is a vital and a widely studied heme-containing enzyme, isolated from a hardy perennial herb, horse radish. It catalyzes the oxidation of various organic and inorganic compounds by using hydrogen peroxide as substrates. HRP is involved in indole-3-acetic acid metabolism, cross-linking of biological polymers and lignification. This enzyme in combination with indole-3-acetic acid or its derivatives acts as a potential therapeutic for targeted cancer therapies. Commercially HRP is used as a constituent of clinical diagnostic kits and is used in immunoassays.


Other Notes


Use of chemically modified peroxidase in benzene; Cross-linking of proteins; Enzyme catalyzed polymerization and precipitation of aromatic compounds from wastewater


Unit Definition


1 U corresponds to the amount of enzyme which oxidizes 1 µmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C

Quality Level100
ManufacturerSIGMA-ALDRICH
Solubility50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA)
Formpowder

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