Pepsin-Agarose from porcine gastric mucosa, lyophilized powder, >=30 units/mg dry solid

Pepsin-Agarose from porcine gastric mucosa, lyophilized powder, >=30 units/mg dry solid

Stock Code: 3579961
Manufacturer Part No: P0609-25KU
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Application


Used to produce F(ab′)2 fragments of antibodies.1


Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG.


Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin.


Biochem/physiol Actions


Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6.


General description


Pepsin is an enzyme found in gastric secretion.


Packaging


10000, 25000, 50000 units in poly bottle


Physical form


Lyophilized powder stabilized with lactose


Quantity


One gram solid swells to 5-10ml packed gel.


Unit Definition


One unit will produce a δA280 of 0.001 per min at pH 2.0 at 37?°C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)

Quality Level200
ManufacturerSIGMA-ALDRICH
Matrix4% cross-linked beaded agarose
Storage Temp.−20°C
Formlyophilized powder

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