MUTANOLYSIN FROM STREPTOMYCES GLOBISPORU

Stock Code: 3581352
Manufacturer Part No: SRE0007-25KU
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Application


Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.


Biochem/physiol Actions


Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.


General description


Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control


Legal Information


Ficoll is a registered trademark of Cytiva


Packaging


25000, 100000 units in poly bottle


Physical form


Lyophilized powder containing Ficoll® and sodium succinate buffer salts


Unit Definition


One unit will produce a δA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Quality Level400
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formlyophilized powder

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