Malic Dehydrogenase from bovine heart, ammonium sulfate suspension, 2000-4000 units/mg protein (modi

Stock Code: 3579894
Manufacturer Part No: M9004-25KU
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Application


Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.


General description


Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.


Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.


Physical form


Suspension in 3 M (NH4)2SO4 - 0.01 M KH2PO4 solution, pH 7.3


Unit Definition


One unit will convert 1.0 µmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Impurities≤0.01% Glutamic-Oxalacetic Transaminase, ≤0.01% Glutamic-Pyruvic Transaminase
Formammonium sulfate suspension

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