Lysozyme from chicken egg white, BioUltra, lyophilized powder, >=98% (SDS-PAGE), >=40,000 units/mg p

Stock Code: 3579818
Manufacturer Part No: L4919-500MG
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£78.28 (exc VAT) per 500MG
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Application


Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.


Lysozyme from chicken egg white has been used: in dielectric spectroscopystudies of dynamics of protein as a control to measure the human lysozyme activity as a supplement in soaking solutionto treat lenses


Biochem/physiol Actions


Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).


Lysozymes participate in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.


Features and Benefits


Highly purified by repeated crystallization and dialysis Each lot is use-tested for isolation of plasmid DNA from E. coli


General description


Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.


Packaging


1, 5 g in poly bottle


500 mg in poly bottle


Preparation Note


3× crystallized


Unit Definition


One unit will produce a δA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, ≥90%
Product LineBioUltra
Formlyophilized powder
Assay≥98% (SDS-PAGE)

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