Lipoprotein Lipase from bovine milk, ammonium sulfate suspension, >=2,000 units/mg protein (BCA)

Stock Code: 3579771
Manufacturer Part No: L2254-1KU
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Application


Lipoprotein Lipase from bovine milk has been used:as a supplement to test its effect on DiI (1,1?-dioctadecyl-3,3,3?-tetramethyl-indocarbocyanine perchlorate)- very-low-density lipoprotein (VLDL) uptake in breast cancer MDA-MB-231 cells to treat human brain microvascular endothelial cells (HBMECs) for the lipolysis of triglyceride-rich lipoproteins (TGRL)to test its effect on gene expression in normal human astrocytes


Biochem/physiol Actions


Lipoprotein Lipase (LPL) from bovine milk contributes to maximal lipolytic activity. It associates with casein micelle. LPL regulates triglyceride utilization and displays positional specificity. Lipases, in general, catalyzes the lipolysis of triglycerides especially at the fatty acid in sn-1 andsn-3 positions of the triglyceride.


General description


Lipoprotein Lipase (LPL) from bovine milk is a glycoprotein. It exists as a homodimer and comprises twoN-linked oligosaccharides. It is heat-labile.


Packaging


5000 units in glass bottle


Physical form


Suspension in 3.8 M ammonium sulfate, 0.02 M Tris HCl, pH 8.0


Preparation Note


Affinity purified


Unit Definition


One unit will release 1.0?nmole of p-nitrophenol per min at pH?7.2 at 37?°C using p-nitrophenyl butyrate as substrate.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formammonium sulfate suspension

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