Lactic Dehydrogenase, recombinant from E. coli, >=90 U/mg

Stock Code: 3578844
Manufacturer Part No: 59747-100MG
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Application


Lactic Dehydrogenase, recombinant fromE. coli has been used:in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assaysto prepare assay buffer to measure pyruvate kinase (PYK) by coupled assayin in vitro DltC D-alanylation assay


Biochem/physiol Actions


Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.


L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD+ to NADH and H+.


General description


LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.


Unit Definition


One unit corresponds to the amount of enzyme which reduces 1 µmol pyruvate per minute at pH 7.4 and 25°C (NADH as cofactor)

Quality Level100
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formpowder

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