L-Lactic Dehydrogenase from bovine heart, Type XVII, buffered aqueous glycerol solution, >=400 units

L-Lactic Dehydrogenase from bovine heart, Type XVII, buffered aqueous glycerol solution, >=400 units

Stock Code: 3579750
Manufacturer Part No: L1006-2.5KU
Order Now for 7 day delivery
£168.54 (exc VAT) per EACH
Quantity: - +

Analysis Note


Protein determined by biuret


Application


L-Lactic Dehydrogenase from bovine heart has been used in ATPase assay of R2 complex Rvb1p-Rvb2p, RecA protein and sarcoplasmic reticulum Ca2+-ATPase (SERCA).


Biochem/physiol Actions


Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.


Variation in the levels of L-Lactic Dehydrogenase (LDH) isoform is a diagnostic marker for tissue damage. The levels of LDH is useful in detecting dairy cattle disease, Mastitis. Dye based purified LDH from bovine heart has analytical applications.


General description


L-Lactic Dehydrogenase (LDH) is present in the cell cytoplasm and is part of the glycolytic pathway. It exists in different isoforms in heart and muscles. LDH is a tetramer corresponding to a molecular weight of 140 kDa. LDH has a nucleotide-cofactor binding site and has the structure of Rossmann fold.


Packaging


12500 units in glass bottle


Physical form


Solution in 50% glycerol containing 0.025 M potassium phosphate buffer, pH 7.5


Unit Definition


One unit will reduce 1.0 µmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formbuffered aqueous glycerol solution

There are no downloads for this product.