L-GLUTAMATE OXIDASE FROM STREPTOMYCES&

Stock Code: 3579495
Manufacturer Part No: G1924-1UN
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Application


L-Glutamate Oxidase from Streptomyces sp may be used:as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor developmentas a component of pencil graphite electrode for biosensing L-glutamate


Biochem/physiol Actions


L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to ?-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.


General description


L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.


This product has a 6xHistidine tag with a thrombin cleavage site.


Unit Definition


One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formsolid

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