Hyaluronidase from sheep testes, Type V, lyophilized powder, >=1,500 units/mg solid

Stock Code: 3579706
Manufacturer Part No: H6254-500MG
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£447.50 (exc VAT) per 500MG
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Application


Hyaluronidase has been used:as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubulesin the pre-treatment of deparaffinized adenomas sections for immunohistochemistryin the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines


Biochem/physiol Actions


These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.


Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively.


General description


Hyaluronidase is present in high amount in testis as it aids fertilization of the mammalian egg. Mammalian hyaluronidase has a catalytic epidermal growth factor (EGF)-like domain and a C-terminal cysteine rich region.


Other Notes


View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer


Packaging


1 g in poly bottle


500 mg in poly bottle


Unit Definition


One unit will cause a change in % transmittance at 600 nm of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 mL reaction mixture (45 minute assay).

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formlyophilized powder

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