Hexokinase from Saccharomyces cerevisiae, Type F-300, lyophilized powder, >=130 units/mg protein (bi

Stock Code: 3579696
Manufacturer Part No: H4502-2.5KU

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Descriptions

Application

Hexokinase has been used:for digesting glucose for measuring glucose content in the root and stem samples of Quercus velutinato treat ADP (adenosine diphosphate) solution along with D-glucose to remove the contaminating ATP (adenosine triphosphate)in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (pH 7.5) for ADP-[13CU]glucose synthesis

Biochem/physiol Actions

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).D-fructose K_M: 0.33 mMD-glucose K_M: 0.12 mMD-mannose K_M: 0.05 mMYeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.Molecular Weight:~54 kDa (monomer)~110 kDa (dimer)Optimal pH: 7.5 to 9.0Extinction Coefficient: E^1% = 8.85 (PI) and 9.47 (PII) at 280 nmActivators: Hexokinase requires Mg²⁺ ions (K_M = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg²⁺ and 4-chloromercuribenzoate)

Packaging

2500, 10000 units in poly bottle

500, 1000 units in glass bottle

Physical form

Lyophilized powder containing approx. 15% sodium citrate

Quality

A mixture of isoenzymes.

Reconstitution

Reconstitute with water or citrate buffer, pH 7.0. Studies have shown excellent stability during repeated freezing and thawing over a period of at least 30 days.

Unit Definition

One unit will phosphorylate 1.0 µmole of D-glucose per min at pH 7.6 at 25 °C.