Heparinase I from Flavobacterium heparinum, Lyophilized powder stabilized with approx. 25% bovine se

Stock Code: 3579675
Manufacturer Part No: H2519-250UN
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Application


Heparinase I from Flavobacterium heparinum has been used:in the digestion of plasma samples to study the effect of medium, washing and sonication on the infectivity of Chlamydia pneumoniain enzymatic removal of heparan sulfate from the surface of human rhabdomyosarcoma cells


Biochem/physiol Actions


Heparinase I from Flavobacterium heparinum acts by cleaving the ?-glycoside linkage at AT III binding site in heparin. This results in small oligosaccharide fragments . Heparinase I neutralizes the protein?s action of anticoagulation. Heparinase I possesses a half-life of 15-18 minutes.


Other Notes


View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer


Unit Definition


One unit (Sigma unit) will form 0.1 µmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formlyophilized solid

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