Glutamic-Pyruvic Transaminase from porcine heart, lyophilized powder, >=75 units/mg protein

Stock Code: 3579643
Manufacturer Part No: G8255-2KU
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Analysis Note


Protein determined by biuret


Application


Glutamic-Pyruvic Transaminase from porcine heart has been used:in the transamination assay with a racemic mixture of synthetic 3-methyl-2-oxoglutarateto remove glutamate from the cell culture medium used for culturing human embryonic kidney (HEK293) cells in the lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction kinetic studies


Biochem/physiol Actions


Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.


Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to ?-ketoglutarate from l-alanine. It plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.


General description


Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver.


Packaging


1000, 2000 units in poly bottle


200 units in glass bottle


Physical form


Lyophilized powder containing citrate and acetate


Unit Definition


One unit will convert 1.0 µmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, 75-95%
Storage Temp.−20°C
Formlyophilized powder

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