Galactosyltransferase from bovine milk, lyophilized powder

Galactosyltransferase from bovine milk, lyophilized powder

Stock Code: 3579557
Manufacturer Part No: G5507-5UN
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Application


Galactosyltransferase from bovine milk has been used: in the generation of hyper-galactosylated variant of IgG1in ? -1,4-galactosyltransferase assay rice root cell extracts in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers


Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.


Biochem/physiol Actions


Galactosyltransferase from bovine milk requires Mn2+ ions for its catalytic functionality. Alteration in the galactosyltransferase levels have been implicated in various diseases states.


Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides.


General description


Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.


Linkage


This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.


Packaging


5, 10 units in serum bottle


25 units in glass bottle


Physical form


Lyophilized powder containing Tris, EDTA, and (NH4)2SO4


Unit Definition


One unit will transfer 1.0?µmole of galactose from UDP-galactose to D-glucose per min at pH?8.4 at 30?°C in the presence of 0.2?mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, 35-65% modified Warburg-Christian
Storage Temp.−20°C
Impurities≤10 % protein α-lactalbumin
Formlyophilized powder

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