Fumarase from porcine heart, ammonium sulfate suspension, 300-500 units/mg protein (biuret)

Fumarase from porcine heart, ammonium sulfate suspension, 300-500 units/mg protein (biuret)

Stock Code: 3579435
Manufacturer Part No: F1757-5KU
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Application


Fumarase from porcine heart has been used:as a positive control in isocitrate dehydrogenase and ?-ketoglutarate dehydrogenase assay for immobilized enzyme based stability assayin fumarate hydratase assays in human lung carcinoma cells (A549)


Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.


Biochem/physiol Actions


Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.


Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.


General description


Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.


Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.


Physical form


Suspension in 3.2 M (NH4)2SO4, 0.05 M KH2PO4, pH 7.5, 0.014 M 2-mercaptoethanol


Preparation Note


Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.


Unit Definition


One unit will convert 1.0 µmole of L-malate to fumarate per min at pH 7.6 at 25 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formammonium sulfate suspension

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