Fructose-6-phosphate Kinase from Bacillus stearothermophilus, Type VII, lyophilized powder, >=50 uni

Fructose-6-phosphate Kinase from Bacillus stearothermophilus, Type VII, lyophilized powder, >=50 uni

Stock Code: 3579429
Manufacturer Part No: F0137-250UN
Order Now for 21 day delivery
£451.29 (exc VAT) per EACH
Quantity: - +

Application


Fructose-6-phosphate kinase from Bacillus stearothermophilus has been used:in steady state analysis of phosphofructokinase activity in the presence of ATP deuterated at the C8 position(C8-D ATP)for standard curve generation for quantifying muscle phosphofructokinase (PFK) activity


Fructose-6-phosphate Kinase from Bacillus stearothermophilus was shown to interact with neuronal nitric oxide synthase (nNOS) causing a defect in glycolytic metabolism and increased fatigability in dystrophic muscle.


Biochem/physiol Actions


Fructose-1,6-bisphosphatase (FBP) is an important enzyme in glucose metabolism. It catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate. Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle.


General description


Fructose-6-phosphate kinase from Bacillus stearothermophilus, a 34 kDa enzyme, belongs to phosphofructokinase (PFK) - like superfamily. The glutamate 161 and arginine 162 residues at the active site are crucial for substrate binding.


Bacillus stearothermophilus phosphofructokinase (BsPFK) is a homotetramer that is allosterically inhibited by phosphoenolpyruvate (PEP), which binds along one dimer-dimer interface.


Packaging


100, 250 units in glass bottle


Physical form


Lyophilized powder containing buffer salt (e.g. phosphate buffer, or Tris buffer with NaCl)


Unit Definition


One unit will convert 1.0 µmole of fructose 6-phosphate and ATP to fructose 1,6-diphosphate and ADP per minute at pH 9.0 at 30 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formlyophilized powder

There are no downloads for this product.