Ficin from fig tree latex, saline suspension, >=1.5 units/mg protein (biuret)

Stock Code: 3579441
Manufacturer Part No: F4125-1KU

Order Now for 7 day delivery

Click Add to Quote for price and delivery

Quantity: - +

Descriptions

Application

Ficin from fig tree latex has been used:in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionalityfor the digestion of eye tissue sections prior to immunostaining and immunofluorescence

Biochem/physiol Actions

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported K_m for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)₂ fragments from mouse IgG1.

General description

Extinction Coefficient: E^1% = 21.0 (280 nm)pI: 9.0

Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges.

Packaging

1000 units in glass bottle

Physical form

Suspension in 2.0 M NaCl and 0.03 M cysteine, pH 5.0

Preparation Note

2× Crystallized

Unit Definition

One unit will produce a δA₂₈₀ of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 mL (1?cm light path).