Deoxyribonuclease II from porcine spleen, Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg p

Stock Code: 3579339
Manufacturer Part No: D4138-80KU
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Application


Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.


DNase II from Sigma has been used to treat transformed cells during the purification of ?-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).


Biochem/physiol Actions


The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3?-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked ?·? heterodimer of approximately 45,000 Da molecular mass.


Packaging


20000, 80000 units in poly bottle


Physical form


Contains sodium chloride


Unit Definition


One Kunitz unit will produce a δA260 of 0.001 per min per mL at pH 4.6 at 25°C; [Mg2+] = 0.83 mM

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, 60-80%
Storage Temp.−20°C
Formlyophilized powder

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