Deoxyribonuclease II from bovine spleen, Type V, essentially salt-free, lyophilized powder, >=1,000
Application
Deoxyribonuclease II from bovine spleen has been used: as a component of digestion mix to isolate immune cells from colon samplesto digest retinal explants for single-cell suspension preparationfor isolation of stromal cells from human colon tissuein the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart
Biochem/physiol Actions
Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3?-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.
Packaging
30000, 150000, 300000 units in poly bottle
Unit Definition
One Kunitz unit will produce a δA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM
| Quality Level | 200 |
|---|---|
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | −20°C |
| Form | essentially salt-free, lyophilized powder |
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