Collagenase from Clostridium histolyticum, release of rat epididymal adipocytes and hepatocytes test

Collagenase from Clostridium histolyticum, release of rat epididymal adipocytes and hepatocytes test

Stock Code: 3579135
Manufacturer Part No: C2139-5G
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Analysis Note


Also contains clostripain, nonspecific neutral protease and tryptic activities.


Application


Collagenase may be used:for the preparation of arterial tissue for the study of advanced glycosylation end products (AGE)for use along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues.in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes.for the preparation of viable hepatocytes from rat liver and for the isolation of fat cells from rat adipose tissue


Biochem/physiol Actions


Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.


Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(?-aminoethyl ether) - N, N, N?,N?-tetraacetic acid, ?-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline. Collagenase enzymes and neutral protease plays an important role in the effective release of cells from tissue. Collagenase recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X represents a neutral amino acid.


General description


Clostridium histolyticum is a pathogenic clostridium that produces crude collagenases. This is a mixture of enzymes containing collagenase, non-specific proteases and clostripain.


Packaging


100, 500 mg in glass bottle


1, 5 g in glass bottle


Unit Definition


One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Suitabilityrelease of rat epididymal adipocytes and hepatocytes tested (for methodology see Type VIII)
Formpowder

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