Choloylglycine Hydrolase from Clostridium perfringens (C.?ßwelchii), lyophilized powder, >=100

Stock Code: 3579191
Manufacturer Part No: C4018-3KU
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£668.57 (exc VAT) per EACH
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Application


The enzyme from Sigma has been used in the analysis of bile samples in various studies.


Biochem/physiol Actions


The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.


Packaging


3000, 7500 units in poly bottle


1500 units in glass bottle


Physical form


Partially purified lyophilized powder containing buffer salts and stabilizer


Unit Definition


One unit will hydrolyze 1.0 µmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, ≥50% biuret
Storage Temp.−20°C
Formlyophilized powder

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