Cholesterol Esterase from Pseudomonas fluorescens, lyophilized powder, >=10,000 units/g protein

Cholesterol Esterase from Pseudomonas fluorescens, lyophilized powder, >=10,000 units/g protein

Stock Code: 3579306
Manufacturer Part No: C9281-500UN
Order Now for 7 day delivery
Click Add to Quote for price and delivery
Quantity: - +

Analysis Note


Protein determined by biuret.


Application


This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.


Cholesterol esterase from Pseudomonas fluorescens has been used in an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase. Cholesterol esterase from Pseudomonas fluorescens has also been used in a study to investigate the nondenaturing protein electrotransfer of the esterase activity of lipolytic preparations.


Biochem/physiol Actions


Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis. Its molecular mass is approximately 129 kDa and the optimum pH range is 7.0-9.0. The enzyme is activated by cholic acid, glycocholic acid, BSA, Mg2+ and 0.3% (v/v) ™ X-100. Ag+, Hg2+ and ionic detergents inhibit the activity of the enzyme.


Legal Information


Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow


Other Notes


Contains potassium phosphate and TRITON® X-100.


Packaging


100, 500 units in poly bottle


Unit Definition


One unit will hydrolyze 1.0 µmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, ~20%
Storage Temp.−20°C
Solubility0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL
Formlyophilized powder

There are no downloads for this product.