Catalase from Aspergillus niger, ammonium sulfate suspension, >=4,000 units/mg protein

Catalase from Aspergillus niger, ammonium sulfate suspension, >=4,000 units/mg protein

Stock Code: 3579168
Manufacturer Part No: C3515-100MG
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£663.00 (exc VAT) per 100MG
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Analysis Note


Protein determined by biuret


Application


Catalase from Sigma has been used as a positive control during the functional characterization of Clostridium difficile spore coat proteins.


Biochem/physiol Actions


Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Isoelectric point is found to be 6.5. Optimum pH for catalytic activity is 7.0. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.


Caution


Freezing of solutions is not recommended.


Packaging


10, 25 mg in glass bottle


Physical form


Suspension in 3.2 M (NH4)2SO4 solution, pH 6.0


Unit Definition


One unit will decompose 1.0?µmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formammonium sulfate suspension

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