Carboxypeptidase G from Pseudomonas sp., lyophilized powder, >=3 units/mg protein

Stock Code: 3579314
Manufacturer Part No: C9658-20UN
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Application


Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO?cGMP signaling in lung adenocarcinoma cells.


Biochem/physiol Actions


Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves ?-glutamyl pteroyl poly-?-glutamate yielding pteroyl-?-glutamate (folic acid) and free glutamate. It is considered highly specific for the ?-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.


Packaging


5 units in poly bottle


20 units in glass bottle


Physical form


Contains sodium acetate salt.


Preparation Note


Chromatographically purified


Solutions should be prepared fresh prior to use.


Unit Definition


One unit will hydrolyze 1.0 µmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.

Quality Level300
ManufacturerSIGMA-ALDRICH
CompositionProtein, ~70% biuret
Storage Temp.−20°C
SolubilityH2O: soluble 0.5 mg/mL
Formlyophilized powder

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