Butyrylcholinesterase from equine serum, lyophilized powder, >=900 units/mg protein

Stock Code: 3579117
Manufacturer Part No: C1057-5KU
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Analysis Note


Protein determined by biuret


Application


Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity has been explored. It has been reported that the level of BChE in human blood correlates to the degree of protection against potentially toxic nerve agents. Cholinesterases have also been investigated for their role in Alzheimer’;s disease.


Biochem/physiol Actions


Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities.BChE can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). The enzyme is activated by Ca2+ and Mg2+. The activity is constant over the pH range of 6.0-8.0. It is inhibited by betaine, nicotine, organophosphates, and carbamate.


Packaging


1000, 5000 units in glass bottle


Physical form


Highly purified; contains buffer salts


Unit Definition


One unit will hydrolyze 1.0?µmole of butyrylcholine to choline and butyrate per min at pH?8.0 at 37?°C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.

Quality Level300
ManufacturerSIGMA-ALDRICH
CompositionProtein, ≥10%
Storage Temp.−20°C
SolubilityH2O: soluble 10 mg/mL
Formlyophilized powder

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