beta-Lactamase from Enterobacter cloacae, Type IV, lyophilized powder, 0.2-0.6 units/mg protein (usi

Stock Code: 3581076
Manufacturer Part No: P4524-250UN
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Analysis Note


Protein determined by biuret.


Application


?-Lactamase from Enterobacter cloacae has been used in the maternal and fetal quantitative blood cultures to avoid any carryover phenomenon. It has also been used as a component in Mueller Hinton (MH) broth for placental cultures.


β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4524 is produced from Enterobacter cloacae and is provided as a lyophilized powder.


Biochem/physiol Actions


β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.


General description


?-Lactamase is a monomeric enzyme of 29 kDa. This product is produced from Enterobacter cloacae and is provided as a lyophilized powder. It acts as a target protein for ?-lactam antibiotics. This enzyme is present in Gram-negative bacteria.


Packaging


100, 250 units in glass bottle


Physical form


Lyophilized powder containing sodium phosphate buffer salts


Unit Definition


One unit will hydrolyze 1.0?µmole of benzylpenicillin per min at pH?7.0 at 25?°C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600?Levy or 75?Pollock units.

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, ~60%
Storage Temp.2-8°C
Formlyophilized powder

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