beta-Glucuronidase from Helix pomatia, Type H-3, aqueous solution, >=90,000 units/mL

beta-Glucuronidase from Helix pomatia, Type H-3, aqueous solution, >=90,000 units/mL

Stock Code: 3579658
Manufacturer Part No: G8885-1ML
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£53.63 (exc VAT) per 1ML
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Application


?-Glucuronidase from Helix pomatia has been used:to convert isoflavone conjugates to their aglycone forms in urine and soymilk samples for high performance liquid chromatography (HPLC) detectionas a component in the protoplasting solutionfor the generation of aromatic and phenolic compounds from the plasma and urine samples for gas chromatography-mass spectrometry (GC-MS)to hydrolyze glucuronide and sulfate conjugated metabolites in fecal samples


?-glucuronidase was used in the measurement of aromatization by a urine technique suitable for the evaluation of aromatase inhibitors in vivo.


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Biochem/physiol Actions


Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis


β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.


General description


β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.


Physical form


Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.


Quality


Many β-glucuronidases derived from mollusks also contain sulfatase activity.


Unit Definition


One Sigma or modified Fishman unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hr at 37?°C at pH 5.0 (30 min assay).


Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 µmole p-nitrocatechol sulfate per hr at pH 5.0 at 37?°C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formaqueous solution

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