beta-Glucuronidase from Escherichia coli, aqueous glycerol solution, >=5,000,000 units/g protein, pH

Stock Code: 3579640
Manufacturer Part No: G8162-25KU

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Descriptions

Application

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

New Technical Article Comparing Performance of Different EnzymesLearn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

?-Glucuronidase from E. coli is used for the enzymatic hydrolysis of b-glucuronides in urine and other fluids. It has a high rate of hydrolytic activity and may be useful for determining the presence of androsterone, 17-hydroxycorticosteroids, and estriol in urine.The optimal conditions for the enzymatic hydrolysis of ?-hydroxytriazolam, one of the major metabolites of triazolam in human urine, were determined using ?-glucuronidase Type IX-A. It is used as a reporter gene in GUS assays to monitor gene expression.

Effective in the hydrolysis of steroid glucuronides.

?-Glucuronidase from Escherichia coli has been used in the enzymatic cleavage and activation of glucuronide prodrugs in non-small cell lung cancer cells, U87 human glioblastoma cell line, in A549 (human lung adenocarcinoma) and KB (human oral squamous carcinoma).

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

?-Glucuronidase catalyzes the hydrolysis of ?-glucuronic acid residues from the non-reducing termini of glycosaminoglycans (GAGs). ?-Glucuronidase is a potential candidate enzyme for gene-mediated enzyme prodrug therapy for glucuronide prodrugs. Use of ?-Glucuronidase-albumin complex based drug delivery could be an effective therapeutic methodology for treating solid tumors.

General description

?-Glucuronidase from Escherichia coli is similar to human glucuronidase enzyme and corresponds to molecular weight close to 69-71 kDa and has an pH optimum of 6.5-7.5. It belongs to family-2 glycosyl hydrolase and has active site residues glutamic acid 394, tyrosine 468 and glutamic acid 504.

Packaging

25000 units in glass bottle

Physical form

Highly purified solution in 50% glycerol

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).