beta-Glucuronidase from Escherichia coli, >20,000,000 units/g protein, recombinant, expressed in E.

Stock Code: 3579477
Manufacturer Part No: G0799-25KU

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Descriptions

Application

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

New Technical Article Comparing Performance of Different EnzymesLearn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

?-Glucuronidase was used in the study of Cer-?-glucuronide synthesis and evaluation of ?-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.

Effective in the hydrolysis of steroid glucuronides.

General description

?-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.

Packaging

25000, 100000 units in poly bottle

Physical form

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Highly purified solution in 50% glycerol

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).