beta-Glucuronidase from Escherichia coli, >20,000,000 units/g protein, recombinant, expressed in E.

Descriptions

Application

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

New Technical Article Comparing Performance of Different EnzymesLearn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

?-Glucuronidase was used in the study of Cer-?-glucuronide synthesis and evaluation of ?-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.

Effective in the hydrolysis of steroid glucuronides.

General description

?-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.

Packaging

25000, 100000 units in poly bottle

Physical form

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Highly purified solution in 50% glycerol

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).