beta-Galactosidase from Escherichia coli, Grade VIII, lyophilized powder, >=500 units/mg protein

beta-Galactosidase from Escherichia coli, Grade VIII, lyophilized powder, >=500 units/mg protein

Stock Code: 3579561
Manufacturer Part No: G5635-5KU
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Application


?-Galactosidase is conjugated to an antibody which specifically recognizes a target molecule (enzyme immunoassay or EIA). ?-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter. It may be used as a positive control protein with anti-?-galactosidase antibodies.


Biochem/physiol Actions


β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.


General description


?-Galactosidase is a tetramer consisting of four equal subunits of 135,000 each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.


Packaging


1000, 3000, 5000, 15000 units in serum bottle


Physical form


Contains Tris buffer salts and magnesium chloride


Physical properties


Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)


Unit Definition


One unit will hydrolyze 1.0 µmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Quality Level300
ManufacturerSIGMA-ALDRICH
CompositionProtein, ≥60% biuret
Storage Temp.−20°C
Formlyophilized powder

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