beta-Galactosidase from Escherichia coli, aqueous glycerol suspension, 600-1,200 units/mg protein (b

beta-Galactosidase from Escherichia coli, aqueous glycerol suspension, 600-1,200 units/mg protein (b

Stock Code: 3579534
Manufacturer Part No: G4155-5KU
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Application


β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.


Biochem/physiol Actions


β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.


Other Notes


Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).


Packaging


5000 units in glass bottle


Physical form


Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4


Physical properties


Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)


Unit Definition


One unit will hydrolyze 1.0 µmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formaqueous glycerol suspension

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