Apyrase from potatoes, ATPase 3.0-40.0 units/mg protein, lyophilized powder

Stock Code: 3578985
Manufacturer Part No: A6132-200UN
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Application


Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .


At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (~10) and another with a low ratio (~1).Reaction: ATP → ADP+Pi → AMP+2Pi.


Biochem/physiol Actions


Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.


Other Notes


Mixture of high and low ratio isoenzymes.


Packaging


Sold on the basis of ATPase units.


200 units in glass bottle


500, 1000, 2000, 5000 units in poly bottle


Physical form


Partially purified, lyophilized powder containing potassium succinate


Unit Definition


One unit will liberate 1.0 micromole of inorganic phosphorus per minute at pH 6.5 at 30 °C

Quality Level300
ManufacturerSIGMA-ALDRICH
CompositionProtein, 30-60%
Storage Temp.−20°C
Formlyophilized powder

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