Aminopeptidase from Aeromonas proteolytica, lyophilized powder, 50-150 units/mg protein

Stock Code: 3579051
Manufacturer Part No: A8200-100UN

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Descriptions

Application

Aminopeptidases are a family of widely distributed proteases, which may be used to study many significant biological processes such as protein maturation, hormone production, and peptide digestion. The enzyme has been used to measure the kinetic rate constant for the binding of bestatin, a general protease inhibitor, to aminopeptidase.

Biochem/physiol Actions

Aminopeptidase from Aeromonas proteolytica is involved in protein maturation, hormone production and peptide digestion.

Aminopeptidase from Aeromonas proteolytica is a metalloenzyme, which contains 2 atoms of Zn²⁺ in a single polypeptide with an approximate molecular weight of 29.5 kDa as determined by sedimentation. This enzyme has a high degree of stability, being stable even at temperatures of 70 °C for several hours. Partial inactivation occurs in 8 M urea. Maximum stability and activity are between pH 8.0-8.5. Aminopeptidase from Aeromonas proteolytica can function as an esterase.

General description

A zinc-containing enzyme.

Packaging

100, 250 units in glass bottle

Physical form

Lyophilized powder containing tricine buffer, pH 8.0, zinc chloride and stabilizer.

Preparation Note

Dissolves in water at 0.9-1.1 mg/mL concentration to form a clear, colorless solution.

Specificity

Catalyzes the release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Unit Definition

One unit will hydrolyze 1.0?µmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH?8.0 at 25?°C.

Specifications

Quality Level	300
Manufacturer	SIGMA-ALDRICH
Composition	Protein, ~40% biuret
Storage Temp.	−20°C
Solubility	H2O: soluble 0.9-1.1 mg/mL, clear, colorless
Form	lyophilized powder