Amidase pseudomonas aeruginosarecombinan t

Stock Code: 3579011
Manufacturer Part No: A6691-1KU
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Application


Amidase from Pseudomonas aeruginosa has been used for testing its capability to hydrolyze ochratoxin A.


The importance of these hydrolases in biotechnology is growing rapidly, because their potential applications span through chemical and pharmaceutical industries as well as in bioremediation. Immobilized amidase can be used efficiently for production of acrylic acid from acrylamide, thus converting a toxic ambient contaminant into widely used industrial raw material. Amidases are potential treatments for human immunodeficiency virus and malaria. They may be used to eliminate metal ions in wastewater .


Biochem/physiol Actions


The amidase from Pseudomonas aeruginosa isa 6 × 38-kDa enzyme that catalyzes the hydrolysis of a small range of short aliphatic amides. Each amidase monomer is formed by a globular four-layer ???? sandwich domain with an additional 81-residue long C-terminal segment .


Physical form


Solution in 50% glycerol containing 7 mM 2-mercaptoethanol and phosphate buffer salt


Unit Definition


One unit will convert 1.0?µmole of acetamide and hydroxylamine to acetohydroxamate and ammonia per min at pH?7.2 at 37?°C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formbuffered aqueous glycerol solution

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