alpha-Mannosidase from Canavalia ensiformis (Jack bean), ammonium sulfate suspension, >=15 units/mg

Stock Code: 3579884
Manufacturer Part No: M7257-25MG

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Descriptions

Application

Liberates mannose from a variety of synthetic and natural α-mannosides.

?-Mannosidase can be used to liberate mannose from a variety of synthetic and natural ?-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ?knock-in? mice.

?-Mannosidase from Canavalia ensiformis (Jack bean) has been used:for the digestion of neutral glycans.to remove O-glycans from recombinant ?-glucosidasefor the inhibition of renal fibroblasts proliferation

Biochem/physiol Actions

??Mannosidase has been shown to inhibit the proliferation of B-lymphocytes.

α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins.

?-Mannosidase cleaves near ?1?2-, ?1?3- and ?1?6-linked mannose residues.

General description

??Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa.

Lysosomal ?-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins.

?-Mannosidase is an exo-glycosidase and an inc metalloenzyme.

Physical form

Suspension in 3.0 M (NH₄)₂SO₄ and 0.1 mM zinc acetate, pH 7.5

Unit Definition

One unit will hydrolyze 1.0 µmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH?4.5 at 25°C.