alpha-Ketoglutarate Dehydrogenase from porcine heart, buffered aqueous glycerol solution, 0.1-1.0 un

Stock Code: 3579740
Manufacturer Part No: K1502-50UN

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Descriptions

Application

?-Ketoglutarate Dehydrogenase from the porcine heart has been used:to study the reversal of nitration by glutathione (GSH) in peroxynitrite-treated cellsto measure its activity by Spectramax M5 microplate spectrofluorimeter using heart mitochondriaas a positive control to evaluate its activity in by Spectramax GEMINI EM fluorescence microplate reader using mice neurons

Biochem/physiol Actions

?-Ketoglutarate dehydrogenase (?-KGDH) is a key enzyme of bioenergetic processes and a controlling unit of metabolic flux through the Krebs cycle or tricarboxylic acid (TCA) cycle. It catalyzes the oxidative decarboxylation of ?-ketoglutarate (KG) to succinyl-CoA by releasing reduced nicotinamide adenine dinucleotide (NADH). It is the rate-limiting reaction of the TCA cycle. This reaction contributes to the electrons of the respiratory chain and requires thiamine pyrophosphate as a cofactor. The reduction of NAD (nicotinamide adenine dinucleotide) is observed to determine its reaction rate. ?-KGDH from porcine has an optimum pH range of 6.6–7.4. This enzyme is inhibited by oxidative stress and results in a metabolic deficiency. However, ?-KGDH is also known to produce reactive oxygen species (ROS) leading to oxidative stress. Defective or limited levels of ?-KGDH cause several neurodegenerative diseases such as Alzheimer?s disease.

General description

?-Ketoglutarate dehydrogenase (?-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2).

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Physical form

Supplied as a 50% glycerol solution containing ~9 mg per mL bovine serum albumin, 30% sucrose, 1.5 mM EDTA, 1.5 mM EGTA, 1.5 mM 2-mercaptoethanol, 0.3% TRITON^™ X-100, 0.003% sodium azide, and 15 mM potassium phosphate, pH?6.8.

Quality

May contain traces of polyethylene glycol.

Unit Definition

One unit will convert 1.0 µmole of β-NAD to β-NADH per min at pH 7.4 at 30 °C in the presence of saturating levels of coenzyme A.