alpha-Glycerophosphate Dehydrogenase from rabbit muscle, Type I, ammonium sulfate suspension, 100-30

Stock Code: 3579592
Manufacturer Part No: G6751-200UN
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Analysis Note


Protein determined by biuret.


Application


?-Glycerophosphate Dehydrogenase from rabbit muscle has been used:in the reaction mixture to measure the glycerol kinase activityto demonstrate compartmentalized enzymatic reactions where NADH is involvedin the reaction mixture assay for L-fuculose-1-phosphate aldolase


Biochem/physiol Actions


?-Glycerophosphate dehydrogenase catalyzes the oxidation of L-?-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.


General description


?-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase.


Physical form


Crystalline suspension in 3.2 M (NH4)2SO4 and 0.1 g/L EDTA solution, pH 6.0


Preparation Note


Prepared by modification of the method of Fondy.


Unit Definition


One unit will convert 1.0 µmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formammonium sulfate suspension

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