alpha-Amylase from human saliva, Type XIII-A, lyophilized powder, 300-1,500 units/mg protein

Stock Code: 3578921
Manufacturer Part No: A1031-5KU
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Application


?-Amylase is used to hydrolyze ? bonds of ?-linked polysaccharides, such as starch and glycogen. Product A1031 is from human saliva, is type IIA, and is supplied as a lyophilized powder. ?-Amylase has been used in various plant studies, such as metabolism studies in Arabidopsis . ?-Amylase from human saliva has been used to study the development of nutraceuticals, which may aid the treatment of diabetes and obesity .


Biochem/physiol Actions


?-Amylase hydrolyzes the ?-(1,4) glucan linkages in polysaccharides of three or more ?-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose.


General description


Human ?-amylase is made up of 496 amino acids in a single polypeptide chain, which is encrypted on chromosome 1. These are produced either in the salivary glands or the pancreas. The salivary and pancreatic ?-amylases have homologous primary sequence but exhibit different cleavage patterns.


We are committed to bringing you?Greener?Alternative Products, which adhere to one or more of The 12 Principles of?Greener?Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.


Other Notes


View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer


Packaging


1000, 5000 units in glass bottle


Physical form


Lyophilized powder containing (NH4)2SO4, sodium citrate and sodium chloride.


Unit Definition


One unit will liberate 1.0 mg of maltose from starch in 3 min at pH?6.9 at 20 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, 5-15% biuret
Storage Temp.−20°C
Formlyophilized powder

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