Acylase I from porcine kidney, Grade II, salt-free, lyophilized powder, 500-1,500 units/mg protein

Acylase I from porcine kidney, Grade II, salt-free, lyophilized powder, 500-1,500 units/mg protein

Stock Code: 3579059
Manufacturer Part No: A8376-1G
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£242.36 (exc VAT) per 1G
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Analysis Note


Protein determined by biuret.


Application


Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids .


Biochem/physiol Actions


Acylase I is a zinc metalloprotein that catalyzes the kinetic resolution of unnatural and rarely occurring ?-amino acids. Its enantioselectivity for the hydrolysis of N-acyl L-?-amino acids is nearly absolute, yet it accepts substrates having a wide range of structure and functionality. Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates.


Packaging


1, 10 g in poly bottle


Unit Definition


One unit will hydrolyze 1.0 µmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formsalt-free, lyophilized powder

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