Achromopeptidase from bacteria, lyophilized powder, >=1,000 units/mg solid

Achromopeptidase from bacteria, lyophilized powder, >=1,000 units/mg solid

Stock Code: 3578959
Manufacturer Part No: A3547-1MU
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Application


Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections.


Biochem/physiol Actions


Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 µn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.


Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.pH Optimum for activity: pH 8.5 - 9Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD600=0.6 over 2 hours at 37 °C.


Packaging


500000, 1000000 units in glass bottle


100000 units in poly bottle


Physical form


crude powder


Unit Definition


One unit will produce a change in A600 of 0.001 per minute per mL at pH 8.0 at 37 °C using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formlyophilized powder

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