Acetate Kinase from Escherichia coli, lyophilized powder, >=150 units/mg protein (biuret)

Acetate Kinase from Escherichia coli, lyophilized powder, >=150 units/mg protein (biuret)

Stock Code: 3579039
Manufacturer Part No: A7437-1KU
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Application


Acetate Kinase from Escherichia coli has been used in assessing acetate kinase activity during the fermatation process of Gluconacetobacter xylinus and in kinetic modeling and steady state studies.


Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Escherichia coli has been used as part of an ATP-regenerating system to study the kinetics of agonist-stimulated transphosphatidylation .


Biochem/physiol Actions


Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA . Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine.


Involved in the metabolism of propanoate, pyruvate and taurine


General description


Acetate kinase belongs to acetate and sugar kinase/Hsc70/actin (ASKHA) structural superfamily. It exists as a dimer and has nucleotide binding site in the cleft between N and C-terminal domain.


Physical form


Lyophilized powder containing trehalose with small amounts of potassium phosphate, magnesium chloride, and dithiothreitol


Unit Definition


One unit will phosphorylate 1.0 µmole of acetate to acetyl phosphate per min at pH 7.6 at 25 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, 5.0-20.0%
Storage Temp.−20°C
Formlyophilized powder

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