Trypsin-Agarose, buffered aqueous suspension, trypsin from bovine pancreas

Stock Code: 3581423
Manufacturer Part No: T4019-100UN
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Application


An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.


General description


The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.


Physical form


Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative


Preparation Note


TPCK-treated


Unit Definition


One unit will hydrolyze 1.0 µmole of BAEE per min at pH 8.0 at 30?°C (titrimetric assay).

Quality Level200
ManufacturerSIGMA-ALDRICH
Matrixbeaded agarose
Storage Temp.2-8°C
Formbuffered aqueous suspension

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