Deoxyribonuclease I bovine, recombinant, expressed in Pichia pastoris, buffered aqueous glycerol sol

Deoxyribonuclease I bovine, recombinant, expressed in Pichia pastoris, buffered aqueous glycerol sol

Stock Code: 3579355
Manufacturer Part No: D5319-2MG
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Application


Deoxyribonuclease I bovine has been used in a study to compare the initial actions of spleen deoxyribonuclease and pancreatic deoxyribonuclease. Deoxyribonuclease I bovine has also been used in a study to investigate deoxythymidine 3′, 5′-di-p-nitrophenyl phosphate as a synthetic substrate for bovine pancreatic deoxyribonuclease.


DNAse I from Sigma was used to treat nuclear lysate to obtain single nucleosomes in a study. The enzyme has also been for the preparation and harvest of mice mammary glands.


Used for the removal of DNA from protein samples.


Biochem/physiol Actions


DNase I is an endonuclease that acts on phosphodiester bonds (adjacent to pyrimidines) to produce polynucleotides with terminal 5?-phosphates. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.


Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5′ phosphates and 3′ OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.


Features and Benefits


RNA purification by removing DNA Prepare DNA for nick translation1 Footprinting assays to determine DNA-protein interactions2


Physical form


Supplied as a solution in 4 mg/ml glycine pH 5.0, 5 mM calcium acetate and 50% glycerol


Preparation Note


Produced without using any animal cells or animal derived materials.


Unit Definition


One unit will produce a δA260 of 0.001 per min per mL reaction mixture using calf thymus DNA at pH 5.0 and 25°C

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formbuffered aqueous glycerol solution

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