Pepstatin A, >=100,000 U/mg

Pepstatin A, >=100,000 U/mg

Stock Code: 3577578
Manufacturer Part No: 77170-50MG
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£653.25 (exc VAT) per 50MG
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Application


Pepstatin A is suitable for use:in the inhibitor cocktail in fluorometric matrix metalloproteinase assayas a protease inhibitor during homogenization of tissueas a protease inhibitor during extraction of cellsto determine the molar concentrations of cathepsin D by titration with pepstatin Ain inhibiting cathepsin DIt can be used in conjunction with E64-d and Leupeptin A to inhibit the degradation of autophagic cargo inside autophagosomes. For this application, the working concentration is typically between 1-10 ?M.


Biochem/physiol Actions


Pepstatin A is an inhibitor of acid proteases (aspartyl peptidases). It forms a 1:1 complex with proteases such as pepsin, renin, cathepsin D, bovine chymosin, and protease B (Aspergillus niger). The inhibitor is highly selective and does not inhibit thiol proteases, neutral proteases, or serine proteases. Solubilized γ-secretase and retroviral protease are also inhibited by Pepstatin A. It has been used to characterize proteases from several sources.


Other Notes


Strong inhibitor of acid proteases - pepsin, cathepsin D, renin


Preparation Note


Pepstatin can be dissolved at 1 mg/ml in 10% (v/v) acetic acid in methanol (9:1 methanol:acetic acid). The inclusion of acetic acid may be necessary to dissolve this peptide in methanol or DMSO. It has been dissolved at 1 to 2 mg/ml in ethanol, but heat may be required for complete dissolution. Solutions of Pepstatin A can be heated as high as 60 °C without any decomposition of the peptide. Stock solutions at 1 mg/ml are stable at least a week at 4 °C. A 1 mM solution in methanol or DMSO is stable for months at -20 °C. If solutions become darker yellow, the reagent is hydrolyzing.A working concentration of 1 µM is stable for at least one day at room temperature.


Unit Definition


1 U corresponds to the amount of inhibitor which reduces the activity of pepsin by 1 U (1 U corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37°C (Hemoglobin, Cat. No. 51290, as substrate, in an endvolume of 16 ml)); 10′000 absorbance-U as described above are equivalent to ∼1 Bergmeyer-U (when 1 pepsin-U is the amount of enzyme which hydrolyzes 1 μmol acetyl-L-phenylalanyl-3,5-diiodo-L-tyrosin per minute at pH 2.0 and 37°C)

Quality Level100
mp233 °C (dec.) (lit.)
InChI keyFAXGPCHRFPCXOO-LXTPJMTPSA-N
InChI1S/C34H63N5O9/c1-17(2)12-23(37-33(47)31(21(9)10)39-34(48)30(20(7)8)38-27(42)14-19(5)6)25(40)15-28(43)35-22(11)32(46)36-24(13-18(3)4)26(41)16-29(44)45/h17-26,30
ManufacturerSIGMA-ALDRICH
Solubilityethanol: soluble 1 mg/mL
Formpowder

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