Nalpha-Tosyl-L-lysine chloromethyl ketone hydrochloride, >=96% (TLC), powder

Stock Code: 3577929
Manufacturer Part No: T7254-500MG

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Descriptions

Application

N?-Tosyl-L-lysine chloromethyl ketone hydrochloride has been used:in chymotrypsin purification to prevent binding of trypsins to the affinity support by inhibiting them in the crude extractas a protease inhibitor in tissue and cell homogenizationas a trypsin inhibitor to determine the specific protease activity levels

Biochem/physiol Actions

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NF-κB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis in cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli.

Caution

Stable at least two years when stored desiccated at −20°C.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Specifications

Quality Level	200
InChI key	YFCUZWYIPBUQBD-ZOWNYOTGSA-N
InChI	1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16;/h5-8,13,17H,2-4,9-10,16H2,1H3;1H/t13-;/m0./s1
Manufacturer	SIGMA-ALDRICH
Form	powder
Assay	≥96% (TLC)