Atto 565 NHS ester, BioReagent, suitable for fluorescence, >=70.0% (coupling to amines)

Atto 565 NHS ester, BioReagent, suitable for fluorescence, >=70.0% (coupling to amines)

Stock Code: 3758638
Manufacturer Part No: 72464-5MG-F
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£1,176.41 (exc VAT) per 5MG
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Features and Benefits


Strong absorption High fluorescence quantum yield High thermal and photo-stability


General description


Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling.


Legal Information


This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.


Other Notes


Fluorescence of bioconjugates; employed in fluorescence resonance energy transfer, FRET, experiments.

Quality Level100
ManufacturerSUPELCO
Product LineBioReagent
Storage Temp.−20°C
Suitabilitysuitable for fluorescence
Formpowder
Assay≥90% (degree of coupling), ≥90% (HPLC)

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